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KMID : 0606920110190010077
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Biomolecules & Therapeutics
2011 Volume.19 No. 1 p.77 ~ p.83
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Sulforaphane Enhances MHC Class II-Restricted Presentation of Exogenous Antigens
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Shin Seul-Mee
Jung Ki-Sung
Park Yoon-Hee
Ko Young-Wook
Lee Chong-Kil
Cho Kyung-Hae
Ha Nam-Joo
Kim Kyung-Jae
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Abstract
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Sulforaphane is an isothiocyanate found in cruciferous vegetables that has been reported to be an effective cancer preventive agent inducing growth arrest and/or cell death in cancer cells of various organs. This paper reports that sulforaphane exerts immunomodulatory activity on the MHC-restricted antigen presenting function. Sulforaphane effi ciently increased the class II-restricted presentation of an exogenous antigen, ovalbumin (OVA), in both dendritic cells (DCs) and peritoneal macrophages in vitro. The class II-restricted OVA presentation-enhancing activity of sulforaphane was also confi rmed using mice that had been injected with sulforaphane followed by soluble OVA. On the other hand, sulforaphane did not affect the class I-restricted presentation of exogenous OVA at concentrations that increase the class II-restricted antigen presentation. At a high concentration (20 μM), sulforaphane inhibited the class I-restricted presentation of exogenous OVA. Sulforaphane did not affect the phagocytic activity of the DCs, and the cell surface expression of total H-2Kb, B7-1, B7-2 and CD54 molecules, even though it increased the expression of I-Ab molecules to a barely discernable level. These results show that sulforaphane increases the class II-restricted antigen presenting function preferentially, and might provide a novel insight into the mechanisms of the anti-cancer effects of sulforaphane.
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KEYWORD
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Sulforaphane, Dendritic cells, MHC class II, Antigen processing
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